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2019, N 3 (37) |
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ECOLOGY
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Gneusheva I., Pavlovskaya N., Solokhina I., Lushnikov A. |
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Biological substantiation of the use of microbial components of Trichoderma spp. in vitro for the recovery of vegetable crops covered ground from phytopathogenic infection |
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Screening of promising antagonist strains of the genus Trichoderma spp. and justification of the use of their microbial components in vitro for the improvement of greenhouse vegetable crops from bacterial and fungal infections. Metabolites of potential antagonists of phytopathogenic microorganisms Trichoderma harzianum, Trichoderma lignorum, Trichoderma viride, Trichoderma lixii; pure cultures of phytopathogenic microorganisms Xanthomonas vesicatoria, Phoma destructiva, Pseudomonas syringae, Ascochyta cucumis. The objects of research were seeds and microplants of tomato variety “Sanka”, cucumber variety “Herman F1”. Metabolites of antagonist strains and phytopathogens were introduced into the medium of growing micro plants of cucumber and tomato (Murasige-Skoog), and the growth-promoting effect of microbial components was determined on the 10th day of vegetation. Determination of antimicrobial activity was performed by the standard disco-diffusion method on pure cultures of phytopathogens. The obtained data showed that the metabolites T. lignorum, T. viride, T. lixii exhibit bactericidal and fungicidal properties in relation to phytopathogenic microorganisms that are common on greenhouse vegetables, while stimulating the growth and development of seedlings and roots of micro-plants. Metabolites T. lixii can be used in the composition of means of protection of vegetable crops of greenhouse as a microbial component, combining immunomodulating properties, bactericidal and fungicidal action.
Keywords: CUCUMBER, TOMATO, PHYTOPATHOGENIC MICROORGANISMS, TRICHODERMA SPP., BACK LENGTH, SPROUT LENGTH, ANTIMICROBIC ACTIVITY |